It is very nice to visit Lambda facility and thanks to warm welcome provided by all the teams. I can easily say one of the best CRO I ever visited. People here are wonderful and doing great job

Reputed Client of Israel

Awesome hospitality & fantastic office. All the best!

Reputed Client of Japan

Excellent hospitality and cooperation received throughout the audit. People are knowledgeable and extremely transparent. Looking for long time relation

Reputed Client of Asia

Facility is too good to conduct the BABE studies and adherence to protocol and quality assurance in conducting studies are meeting sponsor expectations and requirements.

Reputed Client of India

Facility is good, people r trained well and know the subject on which they r working. All the best.

Reputed Client of USA

Highly innovative and advanced research centre that possess bright future for the development of the country.

Reputed International Government Body

It is one of the best facility in India for BA/BE

Reputed Regulatory Authority

Lambda is a very professional CRO. All the staff here are experienced and very knowledgeable. I'm grateful for their accommodations and hospitality. The future, if we want to carry out BE study, we will ask Lambda help again.

Reputed Client of Europe

It was a really pleasant meeting with the PV department, and I am looking forward to the next visit.

Reputed Client of Europe

Amazing and best CRO I ever seen

Reputed Client of China



Home / Blog

Method for the Quantitation of Betahistine in Human Plasma

12 Nov 2018

Betahistine, a vasodilator, is most commonly used to treat the symptoms of Meniere’s disease, vertigo and tinnitus.

Betahistine chemically is 2-[2-(methylamino) ethyl] pyridine and is formulated as the dihydrochloride salt. Betahistine is rapidly converted to its major metabolite 2 pyridyl acetic acid (2-PAA) by monoamino oxidase (MAO) enzymes, and hence surrogate pharmaco-kinetics (PK) of 2-PAA are widely used as measurements for Betahistine PK.

We have developed and validated a novel and highly reproducible bioanalytical method to quantify concentration of Betahistine. Due to the rapid metabolism of Betahistine into 2-PAA by MAO, Betahistine is instable in blood but can be stable in plasma when monoamino oxidase inhibitor (MAOI) are used along with highly controlled low temperature. We have used one of the MAOI to make Betahistine stable in plasma.

Having a low molecular weight and considering a highly polar compound, chromatography separation of Betahistine is also challenging. To achieve low picogram detection and chromatography separation, we used an ion pairing reagent. The current analytical method uses LC-MS/MS analysis of Betahistine in human plasma containing K2EDTA as anticoagulant. The procedure utilizes a simple solid phase extraction and has been developed and validated for a linear range from 7.5 pg/mL to 2000 pg/mL Adequate method sensitivity and selectivity were achieved by monitoring distinct precursor to product ion mass transitions for Betahistine (137.100→94.000) and stable label internal standard Betahistine –d3 (140.100→94.000). This method is highly sensitive, and also selective for its major metabolite 2-PAA.

Looking for a Globally Proven Research Partner?

Contact Us